1X8P
0.85 A Crystal Structure Of Nitrophorin 4 From Rhodnius Prolixus Complexed With Ammonia at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.320, 42.810, 52.920 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 6.000 - 0.850 |
| R-factor | 0.1313 |
| Rwork | 0.131 |
| R-free | 0.15880 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d2u |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.036 |
| Data reduction software | MOSFLM |
| Data scaling software | CrystalClear (D*TREK (MSC/RIGAKU)) |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 0.880 |
| High resolution limit [Å] | 0.850 | 0.850 |
| Rmerge | 0.038 | 0.320 |
| Number of reflections | 113853 | |
| <I/σ(I)> | 18.5 | 1.8 |
| Completeness [%] | 82.8 | 56.8 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 300 | ammonium phosphate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
