1X88

Human Eg5 motor domain bound to Mg-ADP and monastrol

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-12
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9641, 0.9794, 0.9797
Spacegroup name	P 21 21 21
Unit cell lengths	69.706, 79.648, 159.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 1.800
R-factor	0.203
Rwork	0.203
R-free	0.22500
Structure solution method	MAD
Starting model (for MR)	Chain building into electron density map obtained from Solve using MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	93881
Completeness [%]	95.7	73.6
Redundancy	10	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	275	PEG3350, ammonium tartrate, potassium chloride, adenosine diphosphate, monastrol, sodium azide, PIPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 275K
1	VAPOR DIFFUSION, HANGING DROP	6.9	275	PEG3350, ammonium tartrate, potassium chloride, adenosine diphosphate, monastrol, sodium azide, PIPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 275K