1X80
Crystal structure of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 144.403, 144.403, 69.009 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.16122 |
| Rwork | 0.160 |
| R-free | 0.19252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ols |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.074 | 0.378 |
| Number of reflections | 53838 | |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 96.2 | 76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | PEG4000, pH 5.80, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
