1X7T
Structure of TTR R104H: a non-amyloidogenic variant with protective clinical effects
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.033, 85.516, 64.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f86 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.076 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.580 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.097 | 0.281 |
| Number of reflections | 33114 | |
| <I/σ(I)> | 14.2 | 4.9 |
| Completeness [%] | 91.8 | 73.7 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 287 | Ammonium Sulfate, Glycerol, Sodium Acetate, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
