1X7P
Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.881, 76.881, 209.818 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.550 |
| R-factor | 0.20477 |
| Rwork | 0.202 |
| R-free | 0.25653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.426 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.680 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.064 | |
| Number of reflections | 21375 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 300, MES, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
