1X7N
The crystal structure of Pyrococcus furiosus phosphoglucose isomerase with bound 5-phospho-D-arabinonate and Manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.674, 42.382, 57.255 |
| Unit cell angles | 90.00, 120.57, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.890 |
| R-factor | 0.1439 |
| Rwork | 0.141 |
| R-free | 0.21226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PLZ |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.354 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.390 | 1.940 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.052 | 0.349 |
| Number of reflections | 13726 | |
| <I/σ(I)> | 4.5 | 2.79 |
| Completeness [%] | 97.9 | 81.3 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.6M tri-sodium citrate, 50mM 5-phospho-D-arabinonate, 100mM MnCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
