1X7I
Crystal structure of the native copper homeostasis protein (cutCm) with calcium binding from Shigella flexneri 2a str. 301
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-06-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97938, 0.97850, 0.97000, 0.9780 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.327, 97.672, 132.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.830 - 1.700 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.830 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.057 | 0.159 |
| Number of reflections | 52833 | |
| <I/σ(I)> | 8.2 | 4.4 |
| Completeness [%] | 1.0 | 91.7 |
| Redundancy | 4.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG8000, 0.1M Sodium Cacodylate ph 6.0, 0.15M Calcium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
