1X7F
Crystal structure of an uncharacterized B. cereus protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 141.681, 141.681, 141.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.17422 |
| Rwork | 0.171 |
| R-free | 0.23175 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.079 | 0.485 |
| Number of reflections | 22225 | |
| <I/σ(I)> | 30 | 4.95 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris-HCl, 0.2M Sodium Acetate, 0.5 Sodium Chloride, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
