1X6X

Structure 2: cryocolled crystal structure of the truncated pak pilin from Pseudomonas aeruginosa at 0.95A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-06-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.87
Spacegroup name	P 41 21 2
Unit cell lengths	37.577, 37.577, 148.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.510 - 0.960
R-factor	0.1138
Rwork	0.113
R-free	0.13091
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dzo
RMSD bond length	0.019
RMSD bond angle	1.987
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.515	1.010
High resolution limit [Å]	0.950	0.950
Rmerge	0.074
Number of reflections	50494
<I/σ(I)>	21.41
Completeness [%]	75.0	4.5
Redundancy	17.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.2	293	60% Ammonium Sulfate, 0.1M Hepes, pH 8.2, VAPOR DIFFUSION, temperature 293K