1X46
Crystal structure of a hemoglobin component (TA-VII) from Tokunagayusurika akamusi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-30 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.010, 69.110, 99.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.210 - 1.500 |
| Rwork | 0.194 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNX (2000.1) |
| Refinement software | CNX (2000.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.770 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.041 | 0.244 |
| Number of reflections | 23622 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.46 | 12.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG3350, Magnesium Chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
