1X3X

Crystal Structure of Cytochrome b5 from Ascaris suum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	103.225, 41.774, 49.763
Unit cell angles	90.00, 111.13, 90.00

Refinement procedure

Resolution	20.000 - 1.800
Rwork	0.192
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.263
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.051	0.179
Number of reflections	17915
<I/σ(I)>	42.1	9.5
Completeness [%]	96.7	93.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	278	ammonium sulfate, sodium phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K
1	VAPOR DIFFUSION, HANGING DROP	6.8	278	ammonium sulfate, sodium phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K