1X3S

Crystal structure of human Rab18 in complex with Gppnhp

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-08
Detector	MARRESEARCH
Wavelength(s)	0.97931, 0.97917, 0.96860
Spacegroup name	P 1 21 1
Unit cell lengths	37.889, 65.182, 40.710
Unit cell angles	90.00, 91.42, 90.00

Refinement procedure

Resolution	14.970 - 1.320
R-factor	0.185
Rwork	0.185
R-free	0.21000
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	2.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.320	1.320
Number of reflections	44016
<I/σ(I)>	32.5881	7.49867
Completeness [%]	87.2	69.7
Redundancy	5.38303

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	PEG3350, bis-tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K