1X3L
Crystal structure of the PH0495 protein from pyrococccus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-26 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.908, 52.895, 52.683 |
| Unit cell angles | 90.00, 91.31, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1O0U |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.217 |
| Number of reflections | 30781 | |
| <I/σ(I)> | 15.7 | 3.41 |
| Completeness [%] | 99.3 | 97.1 |
| Redundancy | 4.01 | 3.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 295 | ammonium sulfate, acetate, pH 4.6, microbatch, temperature 295K |
