1X2T
Crystal Structure of Habu IX-bp at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.710, 63.509, 66.902 |
| Unit cell angles | 90.00, 117.00, 90.00 |
Refinement procedure
| Resolution | 33.260 - 1.720 |
| Rwork | 0.191 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.100 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.071 | 0.173 |
| Number of reflections | 47809 | |
| <I/σ(I)> | 7.8 | 2.6 |
| Completeness [%] | 99.0 | 92.9 |
| Redundancy | 4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | PEG 1000, PEG 8000, calcium chloride, MES, pH 6.5, MICROBATCH, temperature 293K |
