1X28

Crystal Structure of e.coli AspAT complexed with N-phosphopyridoxyl-L-glutamic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	2003-11-17
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	143.830, 143.830, 81.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.080 - 2.400
R-factor	0.198
Rwork	0.198
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.080	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	36651
Completeness [%]	97.2	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 400, Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K