1X1X

Water-mediate interaction at aprotein-protein interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	200.095, 43.872, 83.234
Unit cell angles	90.00, 110.32, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.191
Rwork	0.191
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.107
Number of reflections	30754
<I/σ(I)>	20.5
Completeness [%]	99.8	99.9
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1