1X1Q
Crystal structure of tryptophan synthase beta chain from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-27 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.187, 109.993, 74.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.500 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.131 | 0.580 |
| Number of reflections | 26974 | |
| <I/σ(I)> | 7.4 | 3.27 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 5.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 291 | 0.2M Magnesium Chloride, 0.1M Tris-HCl, 15 % w/v PEG 4000, pH 8.5, MICROBATCH, temperature 291K |
