1X1P

Crystal structure of Tk-RNase HII(1-197)-A(28-42)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 21 21 2
Unit cell lengths	70.194, 101.258, 42.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.940 - 2.800
R-factor	0.245
Rwork	0.245
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	8435
Completeness [%]	95.0	91.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	200mM MES, 11% PEG8000, 20% Glycerol, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K