1X15
Crystal structure of E. coli transhydrogenase domain I with bound NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.728, 67.089, 76.836 |
| Unit cell angles | 67.22, 80.19, 81.60 |
Refinement procedure
| Resolution | 30.000 - 2.040 |
| R-factor | 0.18888 |
| Rwork | 0.186 |
| R-free | 0.24051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.651 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 40678 | |
| Completeness [%] | 96.4 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | ammonium acetate, trisodium citrate dihydrate, PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
