1X0S
Crystal structure of the 13-cis isomer of bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 101.980, 101.980, 112.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.940 - 2.500 |
| R-factor | 0.271 |
| Rwork | 0.271 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.840 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.056 | 0.500 |
| Number of reflections | 12442 | |
| <I/σ(I)> | 9.2 | 0.5 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 9.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 283 | ammonium sulfate, sodium chloride, citrate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
