1X0K
Crystal Structure of Bacteriorhodopsin at pH 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-28 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 102.450, 102.450, 112.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.960 - 2.600 |
Rwork | 0.263 |
R-free | 0.30900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iw6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.340 | 2.850 |
High resolution limit [Å] | 2.600 | 2.700 |
Rmerge | 0.098 | 0.429 |
Number of reflections | 9845 | |
<I/σ(I)> | 6.7 | 1.8 |
Completeness [%] | 97.9 | 99.4 |
Redundancy | 7.3 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10 | 300 | ammonium sulfate, pH 10.00, VAPOR DIFFUSION, SITTING DROP, temperature 300K |