1X0K
Crystal Structure of Bacteriorhodopsin at pH 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-28 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 102.450, 102.450, 112.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.960 - 2.600 |
| Rwork | 0.263 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.340 | 2.850 |
| High resolution limit [Å] | 2.600 | 2.700 |
| Rmerge | 0.098 | 0.429 |
| Number of reflections | 9845 | |
| <I/σ(I)> | 6.7 | 1.8 |
| Completeness [%] | 97.9 | 99.4 |
| Redundancy | 7.3 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 300 | ammonium sulfate, pH 10.00, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
