1WZX
Crystal Structure of Family 30 Carbohydrate Binding Module.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-29 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.106, 121.106, 122.162 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.830 - 3.520 |
| R-factor | 0.21043 |
| Rwork | 0.206 |
| R-free | 0.29436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wmx |
| RMSD bond length | 0.037 |
| RMSD bond angle | 3.115 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.830 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.147 | 0.405 |
| Number of reflections | 14792 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 94.4 | 83.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.79 | 293 | 0.1M MES, 1.2M Ammonium Sulfate, 10% Dioxane, pH 5.79, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
