1WZV
Crystal Structure of UbcH8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-06 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.231, 35.486, 92.580 |
| Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.20952 |
| Rwork | 0.207 |
| R-free | 0.26726 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1C4Z CHAIN D |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.325 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.046 | 0.237 |
| Number of reflections | 19900 | |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 97.7 | 94.9 |
| Redundancy | 3.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | PEG 3350, calcium acetate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
