1WZN
Crystal Structure of the SAM-dependent methyltransferase from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-26 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0, 0.979480, 0.979085 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.357, 132.011, 145.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.352 |
| Number of reflections | 81819 | |
| <I/σ(I)> | 12.2 | 5.67 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.12 | 6.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.9 | 295 | PEG 3350, Magnesium sulfate, pH 5.9, microbatch, temperature 295K |
