1WZM

Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt R469K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	93
Detector technology	CCD
Collection date	2000-11-27
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	110.663, 115.900, 112.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.530 - 3.200
R-factor	0.216
Rwork	0.216
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	51.530
High resolution limit [Å]	3.200
Number of reflections	24003

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	PEG6000, calcium chloride, Tris, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K