1WZK

Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt D465N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	93
Detector technology	CCD
Collection date	2000-04-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	112.390, 117.660, 112.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.210 - 2.300
R-factor	0.202
Rwork	0.202
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.210
High resolution limit [Å]	2.300
Number of reflections	67154

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	PEG6000, calcium chloride, Tris, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K