1WZC
Crystal structure of Pyrococcus horikoshii mannosyl-3-phosphoglycerate phosphatase complexed with MG2+ and phosphate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9640, 0.9791, 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.833, 70.691, 67.911 |
| Unit cell angles | 90.00, 98.15, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.219 |
| R-free | 0.25270 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 44446 | |
| <I/σ(I)> | 4.06 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 3350, ammonium chloride, tris(hydroxymethyl)aminomethane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 3350, ammonium chloride, tris(hydroxymethyl)aminomethane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
