1WZ3
The crystal structure of plant ATG12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.693, 38.264, 52.511 |
| Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
| Resolution | 28.920 - 1.800 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.24200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.275 |
| Number of reflections | 16031 | |
| <I/σ(I)> | 20.8 | |
| Completeness [%] | 97.3 | 80.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | PEG8000, HEPES, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
