1WYK
SINDBIS VIRUS CAPSID PROTEIN (114-264)
Experimental procedure
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-26 |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.980, 59.540, 71.050 |
| Unit cell angles | 109.40, 101.50, 90.14 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SINDBIS VIRUS CAPSID PROTEIN MUTANT (S215A 106-266) IN TRICLINIC CRYSTAL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.878 * |
| Data reduction software | HKL |
| Data scaling software | HKL |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.028 * | |
| Total number of observations | 53873 * | |
| Number of reflections | 28851 | |
| <I/σ(I)> | 16 | 6 |
| Completeness [%] | 78.3 | 28.8 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.5 (mg/ml) | |
| 10 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 11 | 1 | reservoir | sodium acetate | 150 (mM) | |
| 12 | 1 | reservoir | dioxane | 6 (%(v/v)) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | 12.5 (mM) | ||
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | PEG8000 | 10-13 (%(w/v)) | |
| 6 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 7 | 1 | drop | sodium acetate | 75 (mM) | |
| 8 | 1 | drop | dioxane | 3 (%(v/v)) | |
| 9 | 1 | reservoir | PEG8000 | 20-26 (%(w/v)) |
