1WY5

Crystal structure of isoluecyl-tRNA lysidine synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	81.963, 81.963, 302.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.560 - 2.420
R-factor	0.218
Rwork	0.218
R-free	0.25300
Structure solution method	Crystal 1: Single wavelength protocol, Crystal 2: Mad protocol
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.510
High resolution limit [Å]	2.400	2.420
Number of reflections	39996
<I/σ(I)>	53.1	5.82
Completeness [%]	98.6	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	sodium acetate, sodium formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K