1WXX

Crystal structure of Tt1595, a putative SAM-dependent methyltransferase from Thermus thermophillus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	93
Detector technology	CCD
Collection date	2004-09-27
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9640
Spacegroup name	P 1
Unit cell lengths	77.551, 82.318, 79.616
Unit cell angles	96.34, 90.14, 103.06

Refinement procedure

Resolution	39.550 - 1.800
R-factor	0.203
Rwork	0.203
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wxw
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	328489
Completeness [%]	93.0	70.6
Redundancy	1.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	0.8M NaK Phosphate, 3% sucrose, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K