1WXR

Crystal structure of Heme Binding protein, an autotransporter hemoglobine protease from pathogenic Escherichia coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45PX
Synchrotron site	SPring-8
Beamline	BL45PX
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-04-12
Detector	RIGAKU RAXIS V
Wavelength(s)	0.9794, 0.97925, 1.02
Spacegroup name	P 61 2 2
Unit cell lengths	115.028, 115.028, 437.057
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.360 - 2.200
R-factor	0.20514
Rwork	0.203
R-free	0.24262
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.730
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Number of reflections	81242
Completeness [%]	92.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	PEG 6000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	PEG 6000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K