1WXO
Structure of Archaeal Trans-Editing Protein AlaX in complex with zinc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.472, 97.971, 60.584 |
Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.880 |
Rwork | 0.193 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v7o |
RMSD bond length | 0.005 |
RMSD bond angle | 1.336 |
Data reduction software | TRUNCATE |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.071 | 0.382 |
Number of reflections | 39904 | |
<I/σ(I)> | 10.1 | 3.5 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 5.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG8000, MES-Na, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |