1WXO
Structure of Archaeal Trans-Editing Protein AlaX in complex with zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.472, 97.971, 60.584 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.880 |
| Rwork | 0.193 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v7o |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.336 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.071 | 0.382 |
| Number of reflections | 39904 | |
| <I/σ(I)> | 10.1 | 3.5 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 5.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG8000, MES-Na, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
