1WXI
E.coli NAD Synthetase, AMP.PP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.444, 67.922, 48.394 |
| Unit cell angles | 90.00, 104.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 29229 | |
| Completeness [%] | 91.6 | 87.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.2 | 295 | PEG400, magnesium chloride, sodium-acetate, lithium-meta-tantalate, pH 5.2, VAPOR DIFFUSION, temperature 295K |
