1WX0
Crystal structure of transaldolase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-22 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.693, 142.702, 96.595 |
| Unit cell angles | 90.00, 114.46, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.270 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1L6W.pdb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.108 | 0.548 |
| Number of reflections | 105189 | |
| <I/σ(I)> | 9.5 | 3.59 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 291 | Magnesium Chloride, Tris Hydrochloride, PEG 4000, Glycerol, pH 8.5, MICROBATCH, temperature 291K |
