1WWM

Crystal Structure of Conserved Hypothetical Protein TT2028 from an Extremely Thermophilic Bacterium Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-12-20
Detector	RIGAKU
Wavelength(s)	0.96200, 0.97904, 0.97932
Spacegroup name	P 21 21 21
Unit cell lengths	41.210, 101.121, 102.913
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.650 - 2.610
R-factor	0.234
Rwork	0.234
R-free	0.27500
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	24268

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	MES, PEG8000, magnesium acetate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K