1WWH

Crystal structure of the MPPN domain of mouse Nup35

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-06
Detector	MARRESEARCH
Wavelength(s)	0.9791, 0.9794, 0.9640
Spacegroup name	P 21 21 21
Unit cell lengths	59.486, 104.237, 110.039
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 2.700
R-factor	0.211
Rwork	0.211
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	34813
<I/σ(I)>	14.5	3.2
Completeness [%]	97.8	86.8
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K