1WWC
NT3 BINDING DOMAIN OF HUMAN TRKC RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Collection date | 1996-10-24 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.900, 82.900, 30.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.187 |
| R-free | 0.28800 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.083 |
| Number of reflections | 8088 | |
| Completeness [%] | 94.7 | 89.5 |
| Redundancy | 4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 4MG/ML IN 0.15M NACL, 25MM TRIS PH 7.5, 1MM PMSF VERSUS 25% GLYCEROL, 0.1M NAOAC PH 6.0. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | 0.15 (M) | ||
| 3 | 1 | drop | Tris-HCl | 25 (mM) | |
| 4 | 1 | drop | PMSF | 1 (mM) | |
| 5 | 1 | reservoir | glycerol | 25 (%(v/v)) | |
| 6 | 1 | reservoir | samarium acetate | 0.1 (M) |
