1WW9
Crystal structure of the terminal oxygenase component of carbazole 1,9a-dioxygenase, a non-heme iron oxygenase system catalyzing the novel angular dioxygenation for carbazole and dioxin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9794, 0.9796, 0.9820 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 131.914, 131.914, 131.914 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.940 - 1.950 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.940 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 54782 | |
| Completeness [%] | 98.3 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 15-17.5% MPD, 0.1M MES, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
