1WW5
Agrocybe cylindracea galectin complexed with 3'-sulfonyl lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-08-22 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.623, 57.648, 64.643 |
| Unit cell angles | 89.96, 111.65, 90.14 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | SADIE |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 31157 | |
| <I/σ(I)> | 28.04 | 5.83 |
| Completeness [%] | 94.8 | 93 |
| Redundancy | 2.71 | 1.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ammomium sulfate, lactose, pipes, 3'-sulfonyl lactose, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
