1WW4
Agrocybe cylindracea galectin complexed with NeuAca2-3lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-11-07 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.264, 57.078, 64.306 |
| Unit cell angles | 89.98, 111.73, 90.22 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | SADIE |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 27431 | |
| <I/σ(I)> | 17.65 | 3.6 |
| Completeness [%] | 95.3 | 91.9 |
| Redundancy | 2.94 | 1.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ammomium sulfate, lactose, pipes, N-acetylneuraminyl lactose, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
