1WW1

Crystal structure of tRNase Z from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-11
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	111.902, 73.642, 91.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.800 - 2.600
R-factor	0.225
Rwork	0.225
R-free	0.27900
Structure solution method	Crystal 1: single wavelength protocol, Crystal 2: Mad protocol
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	23807
Completeness [%]	98.8	97.8
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	ammonium phosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K