1WVL

Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	116
Detector technology	IMAGE PLATE
Collection date	2003-04-30
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 2 2
Unit cell lengths	58.650, 58.650, 186.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.276
Rwork	0.230
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1azp
RMSD bond length	0.029
RMSD bond angle	2.508
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	10690
Completeness [%]	99.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6M Tri-Sodium Citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Tri-Sodium Citrate
2	1	1	H2O
3	1	2	Tri-Sodium Citrate
4	1	2	H2O