1WVC
alpha-D-glucose-1-phosphate cytidylyltransferase complexed with CTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2004-08-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96410 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 84.200, 84.200, 157.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tzf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 11780 | |
| <I/σ(I)> | 26.4 | 3.1 |
| Completeness [%] | 97.4 | 90.3 |
| Redundancy | 9.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 400, magnesium chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
