1WVB

Crystal structure of human arginase I: the mutant E256Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-01
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 3
Unit cell lengths	90.272, 90.272, 69.305
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.154
Rwork	0.154
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d3v
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.108
Number of reflections	27626
<I/σ(I)>	12.1	2.7
Completeness [%]	100.0	100
Redundancy	3.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEGMME 2000, Bis-tris, ph 6.5(reservoir), pH 8.5(SAMPLE), VAPOR DIFFUSION, HANGING DROP, temperature 298K