1WV3

Crystal structure of N-terminal domain of hypothetical protein SAV0287 from Staphylococcus aureus

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97938
Spacegroup name	P 21 21 21
Unit cell lengths	45.368, 66.010, 88.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.750
Rwork	0.195
R-free	0.22210
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.436
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	27431
<I/σ(I)>	11.4
Completeness [%]	100.0	100
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	Bis-Tris, PEG3350, Magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K