1WV2
Crystal structure of thiamine biosynthesis protein from Pseudomonas Aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979, 0.97934, 0.97911, 0.97166 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.115, 74.115, 164.947 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.900 |
| R-factor | 0.257 |
| Rwork | 0.254 |
| R-free | 0.30400 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 11974 | |
| Completeness [%] | 98.3 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | Sodium Chloride, Sodium acetate, pH 4.5, VAPOR DIFFUSION, temperature 293.0K |
