1WUQ

Structure of GTP cyclohydrolase I Complexed with 8-oxo-GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-04
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	160.800, 110.957, 70.463
Unit cell angles	90.00, 105.53, 90.00

Refinement procedure

Resolution	40.600 - 2.000
Rwork	0.207
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.054	0.251
Number of reflections	79691
<I/σ(I)>	27.3
Completeness [%]	99.1	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	3.4% PEG400, 0.1M HEPES, 2.0M Ammonium sulfate, 25% glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K