1WUB

Crystal structure of the polyisoprenoid-binding protein, TT1927b, from Thermus thermophilus HB8

Replaces:  1UF6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-05
Detector	MARRESEARCH
Wavelength(s)	0.900
Spacegroup name	C 2 2 21
Unit cell lengths	32.561, 96.948, 109.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 1.650
R-factor	0.22078
Rwork	0.219
R-free	0.26272
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.830
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	21643
<I/σ(I)>	43.8	4.1
Completeness [%]	97.4	86.4
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	1.6M Ammonium Sulfate, 5% Dioxane, 0.1M MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K