1WUA
The structure of Aplyronine A-actin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-14 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.704, 75.123, 67.546 |
| Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
| Resolution | 35.530 - 1.450 |
| R-factor | 0.15266 |
| Rwork | 0.151 |
| R-free | 0.17579 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eqy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.530 |
| High resolution limit [Å] | 1.450 |
| Number of reflections | 69927 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG8000 20%(w/v) 0.1M sodium cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
